Multimessenger Science Opportunities with mHz Gravitational Waves

LISA will open the mHz band of gravitational waves (GWs) to the astronomy community. Thestrong gravity which powers the variety of GW sources in this band is also crucial in a numberof important astrophysical processes at the current frontiers of astronomy. These range fromthe beginning of structure formation in the early universe, through the origin and cosmic evolutionof massive black holes in concert with their galactic environments, to the evolution ofstellar remnant binaries in the Milky Way and in nearby galaxies. These processes and theirassociated populations also drive current and future observations across the electromagnetic(EM) spectrum. We review opportunities for science breakthroughs, involving either direct coincidentEM+GW observations, or indirect multimessenger studies. We argue that for the UScommunity to fully capitalize on the opportunities from the LISA mission, the US efforts shouldbe accompanied by a coordinated and sustained program of multi-disciplinary science investment,following the GW data through to its impact on broad areas of astrophysics. Supportfor LISA-related multimessenger observers and theorists should be sized appropriately for aflagship observatory and may be coordinated through a dedicated mHz GW research center

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Corporate Social Responsibility at African mines: Linking the past to the present

This paper traces the origins of the 'brand' of Corporate Social Responsibility (CSR)employed at large-scale mines across sub-Saharan Africa. Conceived within fortified resource enclaves, the policies adopted and actions taken in the area of CSR at many of the region's large-scale mines today have had had minimal effect on community wellbeing. Further examination reveals that contemporary CSR strategy in the region's mining sector is often a 'repackaging' and 'rebranding' of moves made by major operators during the colonial period and early years of country independence to pacify and engage local communities. Today, this work is being championed as CSR but failing to deliver much change, its impact minimized by the economic and political forces at work in an era of globalization, during which extractive industry enclaves that are disconnected from local economies have been able to flourish. As case study of Ghana, long one of the largest gold mining economies in sub-Saharan Africa, is used to illustrate these points. © 201

Expression of proline-rich Akt-substrate PRAS40 in cell survival pathway and carcinogenesis

Aim: To study the expression of proline-rich Akt-substrate PRAS40 in the cell survival pathway and tumor progression. Methods: The effects of three key kinase inhibitors on PRAS40 activity in the cell survival pathway, serum withdrawal, H 2O 2 and overexpression of Akt were tested. The expression of PRAS40, Akt, Raf and 14-3-3 in normal cells and cancer cell lines was determined by Western blot. Results: The PI3K inhibitors worthmannin and Ly294002, but not rapamycin, completely inhibited the phosphorylation of Akt and PRAS40. The phosphorylation level of Akt decreased after serum withdrawal and treatment with the MEK inhibitor Uo126, but increased after treatment with H 2O 2 at low concentration, whereas none of these treatments changed PRAS40 activity. 14-3-3 is a PRAS40 binding protein, and the expression of 14-3-3, like that of PRAS40, was higher in HeLa cells than in HEK293 cells; PRAS40 had a stronger phosphorylation activity in A549 and HeLa cancer cells than in HEK293 normal cells. In the breast cancer model (MCF10A/MCF7) and lung cancer model (BEAS/H1198/H1170) we also found the same result: PRAS40 was constitutively active in H1198/H1170 and MCF7 pre-malignant and malignant cancer cells, but weakly expressed in MCF10A and BEAS normal cell. We also discussed PRAS40 activity in other NSCLC cell lines. Conclusion: The PI3K-Akt survival pathway is the main pathway that PRAS40 is involved in; PRAS40 is a substrate for Akt, but can also be activated by an Akt-independent mechanisms. PRAS40 activation is an early event during breast and lung carcinogenesis. ©2005 CPS and SIMM